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Image Search Results
Journal: PLoS ONE
Article Title: Severe Blunt Muscle Trauma in Rats: Only Marginal Hypoxia in the Injured Area
doi: 10.1371/journal.pone.0111151
Figure Lengend Snippet: Animals were ventilated with 100% O 2 . The traumatized Musculus gastrocnemius of the trauma group IIa (n = 4 for each time point) and the Musculus gastrocnemius of the control group IIc (n = 6) were harvested and sections of the muscle specimen were analyzed for pimonidazole binding. Hypoxic fraction, percentage area of pimonidazole-labelled muscle cells within an area; injured area, injured area of the traumatized muscle of the trauma group IIa; uninjured area, the adjacent uninjured area of the traumatized muscle of the trauma group IIa; control, tissue area of the non-traumatized muscle of the control group IIc. Values shown represent means ± SEM. *P<0.05 (versus control).
Article Snippet: Immunohistochemical detection of covalently bound pimonidazole was performed according to the manufacturer's protocol for
Techniques: Binding Assay
Journal: PLoS ONE
Article Title: Severe Blunt Muscle Trauma in Rats: Only Marginal Hypoxia in the Injured Area
doi: 10.1371/journal.pone.0111151
Figure Lengend Snippet: The animal was ventilated with 100% O 2 . 450 minutes after trauma, the Musculus gastrocnemius of the traumatized right hind limb was harvested and section of the muscle specimen was analyzed for pimonidazole labelling within the injured area. Injured area is identified by e.g. necrotic muscle cells (arrow) and edema (arrowhead). Hypoxic muscle cells indicated by pimonidazole binding are stained brown (star). Scale bar: 500 µm.
Article Snippet: Immunohistochemical detection of covalently bound pimonidazole was performed according to the manufacturer's protocol for
Techniques: Binding Assay, Staining